Reference Libraries Used in Scaffold DIA

There are three basic reference libraries used by Scaffold DIA for searching results: BLIB, ELIB, and DLIB.


Data-Dependent data searched and then loaded into Scaffold can be exported into a spectral library called a BLIB. Skyline also creates BLIB files. See BLIB specification for more information.


Data-Independent data can be searched in Scaffold DIA and turned into a searchable reference library called a chromatogram library. This kind of library, and ELIB file, holds retention time, intensity, and m/z information. See ELIB specification here for more.


Data-Dependent data can be searched in EncyclopeDIA and turned into a spectral library. See this paper for more information or DLIB spec.

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