Scaffold DDA: Search Engine Quant Support Matrix

Note: Scaffold DDA does support iTRAQ/TMT for all search engines in the broader product sense because these load paths can acquire peaklists/spectra. The MS3 iTRAQ/TMT column below is narrower: it is Yes only when that search-engine import path explicitly populates isobaric quant values.

 

Search engine / import pathLabel-FreeMS3 iTRAQ /TMTSILACNotes
Mascot (direct DAT/MSR import)NoNoNoDirect Mascot readers import identifications and spectra, but no explicit isobaric quant values are populated.
MSFraggerNoNoNo 
FragPipeYesNoNoImports precursor intensity for label-free use, but no explicit isobaric quant values are populated.
SageYesNoNoImports LFQ precursor intensities from Sage LFQ output when present, but no explicit isobaric quant values are populated.
MaxQuant / AndromedaYesYesYes 
Proteome DiscovererYesYesYes 
PD Nodes (via Proteome Discoverer MSF): Sequest, Sequest HT, Amanda, ZCore, Byonic, CHIMERYSYesYesYesProteome Discoverer quant tags are mapped separately from the search engine node.
Generic mzIdentML engines (for example Comet, OMSSA, PEAKS, X! Tandem, Byonic, Sequest)YesNoNoThe mzIdentML reader can load spectra/peaklists, but it does not create explicit isobaric or SILAC quant channels on its own.
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