Prosit-Derived Spectral Libraries for Scaffold DIA Searches

Prosit is a deep learning algorithm developed by the Wilhelm and Kuster group to predict fragmentation and retention times for peptides. We use Prosit to predict DIA-specific libraries from all possible +2H and +3H peptides in FASTA databases. While the search time is the same as other FASTA searches, Scaffold DIA is specifically designed to perform proteome-scale searches, such that Prosit libraries perform as well as smaller sample or tissue-specific DDA libraries used by other tools without the need to perform any DDA library-generation experiments. This approach is particularly powerful when combined with the chromatogram library method to calibrate Prosit libraries for each sample-type and instrument. 

Proteome Software has built a repository of spectral libraries for common organisms which are available below. If you would like us to make a specific Prosit library for you, please contact us with an organism-specific FASTA (with only reviewed entries). Please note that Prosit will only work with carbamidomethylated peptides (C+57). You cannot use any other cysteine alkylation method and you cannot add any PTMs for Prosit other than oxidation.

See this paper for more information.

Library Creation Parameters

The following parameters were used in the conversion process and thus should match your instrumentation settings. If they vary dramatically from your settings please contact us.

Parameter Setting
Charge Range 2 - 3
Maximum Missed Cleavages 1
m/z Range 396.4 - 1002.7
Default NCE 33
Default Charge 3

Available Libraries

Arabidopsis thaliana - 15,896 entries
Download Arabidopsis thaliana DLIB
Download FASTA file

Caenorhabditis elegans- 4,089 entries
Download Caenorhabditis elegans DLIB
Download FASTA file

Danio rerio- 3,125 entries
Download Danio rerio DLIB
Download FASTA file

Drosophila melanogaster - 3,586 entries
Download Drosophila melanogaster DLIB
Download FASTA file

Escherichia coli (strain K-12)- 4,518 entries
Download Escherichia coli DLIB
Download FASTA file

Homo sapiens- 20,350 entries
Download Homo sapiens DLIB
Download FASTA file

Mus musculus- 17,025 entries
Download Mus musculus DLIB
Download FASTA file

Rattus norvegicus- 8,072 entries
Download Rattus norvegius DLIB
Download FASTA file

Saccharomyces cerevisiae- 6,721 entries
Download Saccharomyces cerevisiae DLIB
Download FASTA file 

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